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Capillary Isoelectric Focusing of Proteins

Watch five real proteins self-focus into sharp bands inside a capillary pH gradient: each migrates until its net charge reaches zero at its own isoelectric point, then diffusion and the field balance into a stable peak.

Molecular Biology3DAdvanced60 FPS📱 Mobile-adapted⇄ 2D version

Capillary isoelectric focusing (cIEF) separates proteins not by size but by charge: a stable pH gradient runs the length of a narrow capillary, and each protein migrates under an electric field until it reaches the point where the local pH equals its own isoelectric point (pI) — where its net charge is exactly zero and the driving force vanishes. This simulator models five real proteins spanning pI 1.0 to 10.2 (pepsin, β-lactoglobulin, myoglobin, ribonuclease A and cytochrome c) as they self-focus into sharp bands, with a live pH-dependent net-charge model, thermal diffusion opposing the focusing force, and a real-time detector-style density profile beneath the 3D capillary.

⚙ Under the hood

Watch five real proteins self-focus into sharp bands inside a capillary pH gradient, each migrating until its net charge reaches zero at its own isoelectric point, balanced live against thermal diffusion.

capillary electrophoresisisoelectric focusingprotein separationmolecular biologyelectrophoretic mobilitypH gradient

3D · Three.js / WebGL renderer · 60 FPS target · runs fully client-side, no install