Capillary Isoelectric Focusing — Drift-Diffusion Field Map
A 2D space-time (kymograph) view of capillary isoelectric focusing: five real proteins' concentration profiles are evolved with an explicit finite-volume drift-diffusion solver, the continuum counterpart to particle-based cIEF models, showing each band sharpen toward its isoelectric point in real time.
This is the 2D field-based counterpart to the 3D particle simulator of the same process. Instead of animating individual protein molecules, it solves the drift-diffusion (Nernst-Planck-style) partial differential equation directly for each species' concentration profile along the capillary, using an explicit finite-volume scheme with no-flux boundaries and mass-conserving upwind advection. Five real proteins spanning pI 1.0 to 10.2 (pepsin, β-lactoglobulin, myoglobin, ribonuclease A and cytochrome c) are shown as a scrolling space-time kymograph — position along the capillary on the horizontal axis, elapsed time scrolling down the vertical axis — so you watch each concentration field sharpen into a steady Gaussian band exactly where the local pH equals that protein's isoelectric point, balanced against thermal diffusion in real time.
A 2D space-time kymograph of capillary isoelectric focusing: five real proteins' concentration profiles are evolved with an explicit finite-volume drift-diffusion solver, the continuum counterpart to the 3D particle model, sharpening into steady bands at each protein's own isoelectric point.
2D · HTML5 Canvas 2D · 60 FPS target · runs fully client-side, no install