LNP Gene-Editing Delivery to Hepatocytes

Lipid nanoparticles are the delivery vehicle behind most in-vivo gene-editing and mRNA therapeutics, and getting a payload from the bloodstream into a cell's cytosol is a two-stage bottleneck: first the particle has to slip through the liver's sinusoidal fenestrae to reach a hepatocyte, then it has to escape the acidifying endosome before it is routed to a lysosome and destroyed. This simulator renders that funnel in 3D — particles flow through a sinusoid, attach and get endocytosed, and each one's fate (cytosolic escape or lysosomal degradation) is decided by the same pH-dependent lipid protonation chemistry real formulators tune. Adjust the ionizable lipid's pKa, the N/P charge ratio and particle size to see fenestral uptake, endosomal escape and overall cytosolic delivery yield respond in real time, exactly as they do in an actual LNP formulation screen.