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AAV Episomal Dilution: Vector Genome Loss in a Growing Liver

Interactive 3D hepatocyte-lobule simulator: AAV gene-therapy vector genomes stay episomal and don't replicate, so every liver-cell division randomly splits the copies between daughters. Watch transduced-cell fraction and transgene expression decay as hepatocytes divide, and see why pediatric or regenerating livers lose gene-therapy effect faster than quiescent adult ones.

Rare Disease & Gene Therapy3DAdvanced60 FPS

Adeno-associated virus (AAV) gene therapy delivers its payload as episomal DNA that sits in the nucleus rather than integrating into a chromosome — which is exactly what makes it safe, but also what makes it fade. Because episomes carry no replication origin of their own, every time a transduced hepatocyte divides its vector copies are split randomly between the two daughter cells instead of being doubled first. This simulator renders a small 3D lobule of hepatocytes, each holding its own episomal copy number, and lets you dose the lobule, set a hepatocyte division rate (or fire off a liver-regeneration burst), and watch the transduced-cell fraction and mean copy number decay exactly as the binomial-partitioning math predicts — with a live relative-transgene-expression readout showing why the same AAV dose lasts years longer in a quiescent adult liver than in a growing child's.

⚙ Under the hood

Interactive 3D hepatocyte-lobule simulator: AAV gene-therapy vector genomes stay episomal and never replicate, so every liver-cell division randomly splits the copies between daughters. Set the dose and hepatocyte division rate, or trigger a regeneration event, and watch transduced-cell fraction and transgene expression decay in real time.

gene therapyAAVhepatocyteepisomeliverrare disease

3D · Three.js / WebGL renderer · 60 FPS target · runs fully client-side, no install