AAV Empty/Full Capsid Density-Gradient Separation (2D)

Adeno-associated virus (AAV) gene-therapy lots always contain a mixture of "empty" capsids that failed to package the therapeutic genome and "full" capsids that succeeded — and since only full particles deliver a dose, separating and quantifying the two is a critical manufacturing and quality-control step. This 2D simulator renders a spinning ultracentrifuge tube as a flat, drag-to-rotate cross-section: thousands of empty and full capsid particles start mixed together, then drift under a modeled centrifugal force toward the point in a CsCl/iodixanol density gradient that matches their own buoyant density, exactly as isopycnic banding works in a real AAV purification suite. A live mirrored band histogram and a running resolution-vs-time chart sit alongside the tube view. Tune rotor speed, gradient steepness, run speed and the starting empty:full ratio, and watch live readouts track band resolution, full-capsid purity and recovery as the two populations separate into distinct bands.