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Base Editor Repair Outcome Simulator (2D)

Interactive 2D simulator of cytosine base-editing outcomes: a real editing-window efficiency curve (positions 4-8, peak mid-window), per-base deamination probability, and stochastic DNA-repair outcome sampling across simulated cells.

Molecular Biology2DAdvanced60 FPS📱 Mobile-adapted⇄ 3D version

Cytosine base editing converts a single C:G base pair to T:A without cutting both DNA strands — but the raw chemistry only creates a uracil intermediate, and what the cell does with that uracil determines whether the edit actually lands, and where. This 2D canvas view lays a real 20 nt protospacer plus NGG PAM flat on the screen, highlights the deaminase's real editing window (positions 4-8), and computes a separate, position-dependent deamination probability for every cytosine that window — peaking in the middle and tapering at the edges, exactly as published base-editor efficiency profiles do. Running batches of simulated cells samples every window cytosine independently through the same stochastic repair race as the 3D model (unedited / precise C→T / silent reversion / indel), and a live per-position histogram lets you watch the bell-shaped efficiency curve emerge from the data.

⚙ Under the hood

Simulate the DNA repair race that follows CRISPR cytosine base editing: watch a deaminated uracil intermediate resolve into a precise C→T edit, a silent reversion, or an indel, and see how UGI fusion and strand-selective nicking shift the outcome distribution.

CRISPRbase editingDNA repairmolecular biologygene editing

2D · HTML5 Canvas 2D · 60 FPS target · runs fully client-side, no install