Nanotopography Stem Cell Fate Simulator

A colony of mesenchymal stem cells sits on a nanoscale substrate whose geometry and stiffness you control. Switch between a flat surface, nanogrooves and a nanopillar array, tune the feature spacing and height, and slide substrate stiffness from soft to bone-hard — and watch each cell's cytoskeletal tension, spread footprint and elongation update live as it commits to an osteogenic, myogenic, neurogenic or adipogenic fate, driven by a simplified composite of the published mechanotransduction cues (Engler stiffness response, McBeath/Kilian RhoA-tension fate switch, Yim nanogroove-induced neuronal elongation, Dalby nanopillar-driven osteoinduction).