Home▸Molecular Biology▸Pathway Over-Representation Analysis (2D)

Pathway Over-Representation Analysis (2D)

Interactive 2D pathway over-representation analysis: watch significant and pathway genes settle into a physics-driven Venn diagram, then read the exact hypergeometric (Fisher's exact test) enrichment p-value live, cross-checked against a Monte Carlo null distribution.

Molecular Biology2DAdvanced60 FPS📱 Mobile-adapted⇄ 3D version

Details

CategoryMolecular Biology

RenderingCanvas 2D

DifficultyAdvanced

Target FPS60

ControlsChoose a correlation preset (Enriched, Depleted, or Random/independent), drag sliders for the significant-gene count K and pathway-set size M, click New Random Draw to resample, and Monte Carlo Null (3000x) to empirically confirm the exact hypergeometric p-value.

bioinformaticsgene expressionover-representation analysishypergeometric testFisher's exact testpathway analysisgenomics

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Over-representation analysis is the other standard pathway-analysis method used across genomics, alongside rank-based approaches like GSEA: rather than walking a ranked list, it draws a hard line between "significant" and "not significant" genes and asks whether a pathway's members land on the significant side more often than chance predicts. This simulator builds a background population of genes, lets you choose how strongly pathway membership correlates with significance, and computes the real hypergeometric (Fisher's exact test) enrichment p-value live — visualized as a physics-driven Venn diagram whose circles emerge from real spring and repulsion forces, paired with the exact probability distribution and a Monte Carlo null check.

⚙ Under the hood

Watch significant and pathway genes settle into a real physics-driven Venn diagram, then read the exact hypergeometric (Fisher's exact test) enrichment p-value live, cross-checked against a Monte Carlo null distribution — the classic over-representation counterpart to rank-based GSEA.

bioinformaticsgene expressionover-representation analysishypergeometric testFisher's exact testpathway analysisgenomics

2D · HTML5 Canvas 2D · 60 FPS target · runs fully client-side, no install