Twin Prime Editing (twinPE) — 2D Strand View

Twin prime editing installs an edit using two pegRNAs at once, each nicking one strand of the DNA duplex and reverse-transcribing its own new 3' flap. When the two flaps share a homology arm they anneal to each other instead of the original genomic strand, replacing the whole interval between the nicks — insertions, deletions and precise replacements far larger than a single-flap prime edit can reach, and without a double-strand break. This 2D simulator draws the duplex as a linear side-view ladder, with the two nick sites and their growing flaps rendered along it; tune the homology arm, the size of the edit, the nick-to-nick spacing and the RT variant (PE2 / PE2max / PEmax) and watch each attempt either anneal into an installed edit or dissociate, with a live efficiency estimate driven by the same three determinants that govern real twinPE outcomes. Drag the canvas to pan and scroll (or pinch) to zoom.