Home▸Molecular Biology▸Organoid Flow Cytometry Sorter — 2D Cross-Section

Organoid Flow Cytometry Sorter — 2D Cross-Section

A 2D side-view cross-section of a virtual flow cytometer: dissociate an intestinal organoid into single cells, set FSC and fluorescence gates, and watch electrostatic-deflection cell sorting in a pannable, zoomable 2D scene.

Molecular Biology2DModerate60 FPS📱 Mobile-adapted⇄ 3D version

Details

CategoryMolecular Biology

RenderingCanvas 2D

DifficultyModerate

Target FPS60

ControlsSliders for stem/progenitor fraction, flow rate, deflection voltage, FSC debris-exclusion gate and FL1 marker gate; buttons to switch the fluorescent marker channel (LGR5-GFP vs Villin-RFP), pause/resume the stream, and reset counters; drag the main scene to pan and scroll (or pinch) to zoom.

flow-cytometrycell-sortingorganoidmolecular-biologyfacselectrostatic-deflection2d-cross-section

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Dissociate a dissociated intestinal organoid into single cells and watch them stream through a 2D side-on cross-section of a virtual flow cytometer. Each cell carries a forward-scatter (size) value and a fluorescence value on whichever marker channel you select — LGR5-GFP for stem/progenitor cells or Villin-RFP for differentiated cells — sampled from the organoid's own composition. Set a debris-exclusion gate on size and a marker-intensity gate on fluorescence, and watch the real electrostatic-deflection physics of a droplet cell sorter route each cell into a positive, negative or waste collection tube, with a live FSC-vs-FL1 scatter plot and running purity/yield statistics exactly like real flow-cytometry analysis software. Drag the main view to pan and scroll (or pinch) to zoom.

⚙ Under the hood

A 2D side-view cross-section of a virtual flow cytometer: dissociate an intestinal organoid into single cells carrying forward-scatter (size) and fluorescence-marker values, set FSC and FL1 gates, and watch electrostatic-deflection cell-sorter physics route each cell into a positive, negative or waste tube with a live scatter plot and purity/yield stats. Drag to pan and scroll to zoom the scene.

flow-cytometrycell-sortingorganoidmolecular-biologyfacselectrostatic-deflection2d-cross-section

2D · HTML5 Canvas 2D · 60 FPS target · runs fully client-side, no install