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Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS)

Interactive 3D HDX-MS simulator: watch deuterium uptake spread across a folded biologic's backbone over exposure time, compare native vs destabilized conformations, and see which peptide segments a comparability study would flag.

Structural Biology & Biophysics3DAdvanced60 FPS

Hydrogen-deuterium exchange mass spectrometry probes a biologic's higher-order structure (HOS) by soaking it in heavy water and measuring how fast backbone amide hydrogens swap for deuterium — buried, hydrogen-bonded regions exchange slowly while flexible surface loops exchange almost instantly. This simulator renders a folded backbone in 3D, colors each peptide segment by its live %D uptake as exposure time advances on a log scale, and lets you toggle between a native fold and a destabilized conformation to see exactly which segments a real comparability study would flag as significantly perturbed — the core analytical technique referenced in biologics HOS and comparability assessment.

⚙ Under the hood

Watch deuterium uptake spread across a folded biologic's backbone over exposure time in 3D, and compare a native fold against a destabilized conformation to see which peptide segments an HDX-MS comparability study would flag.

HDX-MSbiologicsprotein structurecomparabilitymass spectrometrybiophysics

3D · Three.js / WebGL renderer · 60 FPS target · runs fully client-side, no install