Home▸Nanotechnology & MEMS▸DNA Molecular Beacon Biosensor

DNA Molecular Beacon Biosensor

Interactive 2D FRET biosensor simulator: a schematic hairpin DNA molecular beacon opens and closes according to a real coupled thermodynamic equilibrium (stem melting + target hybridization), with live fluorescence readout and a genuine sigmoidal dose-response curve emerging from the actual free-energy calculation.

Nanotechnology & MEMS2DAdvanced60 FPS📱 Mobile-adapted⇄ 3D version

Details

CategoryNanotechnology & MEMS

RenderingCanvas 2D

DifficultyAdvanced

Target FPS60

ControlsSliders set target DNA concentration, hybridization temperature and ionic strength (NaCl); a Reset assay button reseeds the schematic probe population and restarts the timer; Probe view/Dose-response buttons toggle between the animated hairpin scatter (each probe's open/closed draw sampled from the live equilibrium fraction) and a live fluorescence-vs-log[target] dose-response curve traced from the same thermodynamic model.

biosensorDNAFRETfluorescencediagnosticsnanotechnology

Related simulations

Lateral Flow Nanoparticle AssayWatch a liquid sample wick by capillary action through a lateral-flow test strip, carrying…

Nanowire Biosensor: Reversible Binding Chain (2D)A real per-site stochastic reversible Langmuir binding simulation (individual molecules bind…

Silicon Nanowire FET Biosensor (2D)2D cross-section of a label-free silicon-nanowire FET biosensor: charged biomarkers diffuse,… ⊞ All 351 in Nanotechnology & MEMS →

A molecular beacon is a hairpin DNA probe that stays dark until it finds its target. This 2D simulator solves the same real biochemistry as a closed-form thermodynamic equilibrium: a coupled stem-melting/target-hybridization free energy sets the exact fraction of the probe population sitting open and fluorescent at any instant, driving a schematic hairpin's open/close animation, a live fluorescence readout, and a genuine sigmoidal dose-response curve traced directly from the same free-energy calculation as you sweep target concentration. Tune target concentration, hybridization temperature and ionic strength to see how real diagnostic assays trade off sensitivity and background.

⚙ Under the hood

Watch a population of hairpin DNA molecular beacons flicker between a FRET-quenched closed state and a fluorescent open state, capturing a complementary target strand and lighting up permanently — with real Forster-distance quenching and target-concentration-driven hybridization kinetics.

biosensorDNAFRETfluorescencediagnosticsnanotechnology

2D · HTML5 Canvas 2D · 60 FPS target · runs fully client-side, no install