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TMT Isobaric Labeling: Reporter-Ion Quantification

Interactive 3D simulator of tandem mass tag (TMT) isobaric labeling: watch multiplexed peptide channels co-isolate as one MS1 precursor peak, then fragment into distinct MS2 reporter ions whose intensities reveal relative protein abundance, complete with shot-noise and co-isolation ratio-compression.

Molecular Biology3DAdvanced60 FPS📱 Mobile-adapted⇄ 2D version

Tandem mass tags (TMT) let a proteomics lab pool up to several digested samples into one LC-MS/MS run: because each tag's reporter and balance groups sum to the same mass, tagged copies of the same peptide from every sample become chemically isobaric and are selected together as a single MS1 precursor peak. This simulator renders that peak as a cluster of colored ion particles, then lets you trigger HCD fragmentation to watch it burst into six distinct reporter-ion channels whose bar heights encode each sample's relative protein abundance. Sliders control the true abundance in each channel, how much co-isolated interference contaminates the isolation window (causing real-world ratio compression), and how deep the instrument samples ions (shot noise) — live readouts compare the measured ratios against the ground truth so you can see exactly how much of the difference is measurement error.

⚙ Under the hood

Simulate tandem mass tag (TMT) multiplexed proteomics: six isobarically labeled sample channels co-isolate as one MS1 precursor peak, then fragment into distinct reporter ions whose intensities quantify relative protein abundance, including realistic shot noise and co-isolation ratio compression.

proteomicsmass spectrometryTMTquantificationisobaric labelingbiochemistry

3D · Three.js / WebGL renderer · 60 FPS target · runs fully client-side, no install