Home▸Molecular Biology▸TMT Reporter-Ion Mass Spectrum: 2D Peak-Fitting View

TMT Reporter-Ion Mass Spectrum: 2D Peak-Fitting View

Interactive 2D mass-spectrum simulator of tandem mass tag (TMT) isobaric labeling: watch the MS1 precursor isotope envelope (averagine + Poisson C-13 statistics, Orbitrap resolution scaling) collapse into a resolved MS2 reporter-ion spectrum, with live shot-noise and co-isolation ratio-compression math.

Molecular Biology2DAdvanced60 FPS📱 Mobile-adapted⇄ 3D version

Details

CategoryMolecular Biology

RenderingCanvas 2D

DifficultyAdvanced

Target FPS60

ControlsSix per-channel abundance sliders, a co-isolation interference slider, an MS2 ion-sampling-depth (AGC) slider, a precursor charge-state selector, and a Fragment (HCD) trigger that sweeps the plotted spectrum from the MS1 isotope envelope to the six resolved MS2 reporter-ion peaks.

proteomicsmass spectrometryTMTquantificationisobaric labelingbiochemistrymass spectrumisotope envelope

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Tandem mass tags (TMT) let a proteomics lab pool several digested samples into one LC-MS/MS run: because each tag's reporter and balance groups sum to the same mass, tagged copies of the same peptide from every sample become chemically isobaric and appear together as a single MS1 precursor isotope envelope. This 2D companion plots the instrument's actual spectrum rather than an animated 3D scene: the precursor's isotope comb is built from an averagine elemental model and Poisson ¹³C statistics, drawn at an Orbitrap-realistic peak width that narrows with mass, then — on Fragment — sweeps into six resolved reporter-ion peaks whose heights encode each sample's relative protein abundance. Sliders control the true abundance in each channel, how much co-isolated interference contaminates the isolation window (causing real-world ratio compression), and how deep the instrument samples ions (shot noise); live readouts compare the measured ratios against ground truth.

⚙ Under the hood

2D mass-spectrum companion to the TMT isobaric labeling simulator: plots the MS1 precursor isotope envelope from an averagine elemental model and Poisson carbon-13 statistics at Orbitrap-realistic peak width, then sweeps into a resolved MS2 reporter-ion spectrum with live shot-noise and co-isolation ratio-compression math.

proteomicsmass spectrometryTMTquantificationisobaric labelingbiochemistrymass spectrumisotope envelope

2D · HTML5 Canvas 2D · 60 FPS target · runs fully client-side, no install