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CRISPR-Cas9 Gene Editing: Target Search & Repair

Interactive CRISPR-Cas9 simulator: scan a DNA strand for PAM sites, watch Cas9 lock onto a guide-RNA target, cut both strands, and see the cell repair the break by error-prone NHEJ or precise HDR.

Genetics & Evolution2DModerate60 FPS📱 Mobile-adapted

This simulation walks through the real mechanics of a CRISPR-Cas9 edit on a 3D DNA double helix. Cas9 sweeps the strand for PAM (NGG) sites, checks each candidate against your 20-nucleotide guide RNA with an adjustable mismatch tolerance, locks onto the best match, and cuts both strands three base pairs upstream of the PAM. You then choose the cell-cycle phase and whether a donor template is available, and watch the break get resolved by error-prone NHEJ (random insertions/deletions, with a live frameshift check) or precise HDR (exact template-directed correction) — with off-target cuts tracked separately, exactly as researchers must account for them in real gene-editing therapies.

⚙ Under the hood

This simulation allows you to explore the core concepts of CRISPR-Cas9 gene editing technology. Manipulate DNA sequences and observe the effects on simulated organisms, gaining a deeper understanding of this revolutionary biotechnology.

Gene EditingBiotech

2D · HTML5 Canvas 2D · 60 FPS target · runs fully client-side, no install