Biomarker ELISA: Antibody Binding Kinetics & Detection Limit

Validating a candidate biomarker for diagnostic use comes down to one question: can an antibody-based assay actually detect it, reliably, at the concentrations that matter? This simulator builds a real sandwich-ELISA dose-response titration — eight wells spanning a two-fold antigen dilution series plus a blank — and drives each well's signal with pseudo-first-order antigen-antibody binding kinetics, so the plate develops in 3D as incubation time advances rather than jumping straight to equilibrium. Reagent concentrations (capture-antibody density, binding affinity, nonspecific background) and incubation time are all live controls, and the readout panel computes the two numbers a real assay validation report leads with: the apparent EC50 read off the curve and the limit of detection set by assay noise — both compared directly against the true underlying affinity so you can see exactly how incubation time and reagent choice bias what the assay reports.