🦠 Postbiotic Metabolite Health Effect Simulator

Dietary Fiber Reaches the Colon

Fermentable fiber survives digestion and arrives intact in the colon.

• 25–38 g: Daily fiber target (adult recommendation)
• ~0%: Fiber digested in small intestine (passes through undigested)
• Inulin, resistant starch: Fermentable fiber types (main substrates)
• 4–8 h: Transit time to colon (after a meal)

Fiber resists upper-gut digestion

Human enzymes cannot break fiber's bonds.

Substrate variety matters

Different fibers feed different bacterial species.

Low intake starves the colon

Low fiber diets reduce metabolite output sharply.

Bacteria Ferment Fiber Anaerobically

Trillions of resident bacteria break fiber down without oxygen.

• 10¹¹–10¹²: Colon bacterial density (cells per gram)
• Anaerobic: Fermentation environment (oxygen-free colon lumen)
• Faecalibacterium, Roseburia: Key genera (major fermenters)
• Within hours: Fermentation onset (of fiber arrival)

Cross-feeding networks

Bacterial species exchange fermentation byproducts cooperatively.

Capacity varies by person

Microbiome diversity determines fermentation efficiency.

Fiber shapes the community

Regular fiber intake favors beneficial fermenting bacteria.

Short-Chain Fatty Acids Are Generated

Fermentation yields butyrate, propionate, and acetate as byproducts.

• 60:20:20: Typical colon SCFA ratio (acetate:propionate:butyrate)
• ~200–800 mmol: Total SCFA produced daily (in the colon)
• Colonocyte fuel: Butyrate primary role (local energy source)
• Liver metabolism: Propionate primary role (gluconeogenesis signal)

Three main SCFAs

Acetate, propionate, and butyrate dominate production.

Independent of live bacteria

Metabolites act even after bacteria are gone.

Concentration follows fiber intake

More fermentable fiber yields more SCFA output.

Butyrate Fuels the Colonocyte

Colon cells burn butyrate first, before glucose or fat.

• ~70%: Colonocyte energy from butyrate (of total energy needs)
• Mitochondria: Butyrate oxidation site (beta-oxidation pathway)
• Supports renewal: Effect on cell turnover (healthy epithelial cycle)
• Cell starvation: Butyrate deficiency effect (colonocyte energy gap)

Preferred fuel source

Colonocytes prioritize butyrate over glucose.

Sustains the epithelial lining

Steady fuel keeps colon cells renewing properly.

Low butyrate weakens cells

Energy-starved colonocytes function poorly overall.

SCFAs Support Systemic Health

Metabolites strengthen the gut barrier and calm inflammation body-wide.

• Reinforced: Tight junction effect (reduced gut permeability)
• Reduced: Inflammatory marker effect (lower cytokine signaling)
• Bloodstream: Propionate systemic route (liver and immune signaling)
• Improved: Barrier integrity outcome (with adequate SCFA supply)

Barrier reinforcement

SCFAs tighten junctions between colonocytes.

Anti-inflammatory signaling

SCFAs dampen immune inflammatory pathways.

Effects reach beyond the gut

Absorbed metabolites influence liver and immune organs.