Target-Decoy FDR Control in Proteogenomics (2D)

Proteogenomic pipelines match mass-spectrometry spectra against protein databases built from a sample's own transcriptome, and every match carries a real risk of being pure noise. This simulator generates two synthetic populations of peptide-spectrum matches — true target hits and null decoy hits — from the same statistical model real search engines produce, lets you inflate the search space with a larger database or a wider post-translational-modification search, and renders the result across three linked 2D panels: a live score histogram with a draggable acceptance cutoff, a full FDR-versus-threshold curve so you can see the whole trade-off at once, and a pan/zoomable scatter strip of individual matches. Watch the false discovery rate update in real time as you trade sensitivity for confidence, exactly the trade-off that decides which peptides are trustworthy enough to call as neoantigens.