Thermal Proteome Profiling: Drug Target Melt-Shift Simulator

Thermal proteome profiling heats a protein population, removes whatever aggregates by centrifugation, and reads out the soluble fraction left behind — the same read-out mass spectrometry captures at proteome scale. This simulator renders a target protein population and an unrelated off-target control side by side as instanced 3D spheres, each carrying its own randomly offset melting threshold so the group denatures along a real sigmoidal curve rather than all at once. Add a ligand at a chosen concentration and binding affinity and watch the target's apparent melting temperature shift upward via a saturating Langmuir isotherm, while the control — which the drug does not bind — stays put, exactly the specificity signature that identifies a genuine drug target in a real TPP/CETSA screen.