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Cofactor Recycling Loop: NADPH Regeneration in Biocatalysis

Interactive 3D model of enzymatic cofactor recycling: a ketoreductase consumes NADPH while a coupled glucose dehydrogenase regenerates it, letting a catalytic trace of cofactor turn over a full-size substrate pool. Tune loading, recycling capacity, temperature and glucose feed live.

Biocatalysis Green Enzyme Synthesis3DAdvanced60 FPS

Industrial biocatalysis rarely buys its cofactors by the mole — instead a second, coupled enzyme regenerates the expensive reduced cofactor in place, so a catalytic trace turns over the whole substrate pool. This simulator models that loop directly: a ketoreductase site consumes NADPH to make product while a glucose dehydrogenase site spends glucose to regenerate it, with real Michaelis-Menten kinetics, a Q10 temperature rule and a live cofactor turnover-number readout driving a 3D shuttle of cofactor particles between the two active sites.

⚙ Under the hood

Watch a ketoreductase consume NADPH to form product while a coupled glucose dehydrogenase regenerates it from glucose, letting a catalytic trace of cofactor turn over the whole substrate pool. Tune cofactor loading, recycling-enzyme ratio, temperature and glucose feed and watch conversion, turnover number and redox balance respond live.

biocatalysisenzyme kineticscofactor recyclingNADPHgreen chemistryketoreductase

3D · Three.js / WebGL renderer · 60 FPS target · runs fully client-side, no install