Home▸Nanotechnology & MEMS▸Single-Molecule Localization Microscopy: STORM/PALM Super-Resolution

Single-Molecule Localization Microscopy: STORM/PALM Super-Resolution

Interactive 3D single-molecule localization microscopy simulator: switch between conventional wide-field imaging, where densely packed fluorophores blur into one indistinguishable smear, and STORM/PALM-style stochastic blinking that isolates and precisely localizes molecules one sparse subset at a time, accumulating a super-resolved image far below the diffraction limit.

Nanotechnology & MEMS3DAdvanced60 FPS💨 Air & Wind

Ordinary fluorescence microscopy cannot resolve two structures closer together than the diffraction limit — their blurry point-spread-function blobs overlap into one indistinguishable smear. STORM/PALM-style localization microscopy instead blinks on a sparse random subset of molecules at a time, precisely fits each isolated blob's center, and accumulates thousands of these pinpoint localizations into a final image resolved far below the diffraction limit.

⚙ Under the hood

Two fluorescent filaments run 130nm apart — inside the ~250nm diffraction limit. Light every molecule at once and their blurry point-spread-function blobs merge into one indistinguishable smear; switch to STORM/PALM mode and only a sparse random subset blinks on each frame, each isolated blob is localized to a pinpoint, and thousands of accumulated localizations resolve the two filaments distinctly.

super-resolution microscopySTORMPALMsingle-molecule localizationdiffraction limitpoint spread functionfluorescence imagingnanotechnology

3D · Three.js / WebGL renderer · 60 FPS target · runs fully client-side, no install