This simulator demonstrates how surface plasmon resonance responds in real time to molecular binding events at a gold sensor surface within the Kretschmann prism-coupling configuration, showing how the reflectance dip angle shifts as analyte molecules associate with and dissociate from immobilized receptors, and how these shifts trace out a realistic sensorgram curve.
Adjust the angle of incident light to locate the resonance dip in the reflectance curve, then start an analyte injection to watch the resonance angle shift as binding accumulates on the gold surface. Switch back to buffer flow to observe dissociation, and vary analyte concentration or the association and dissociation rate constants to see how they reshape the resulting sensorgram trace.
Controls include the angle of incident light on the prism, analyte concentration, association rate constant, dissociation rate constant, and an injection or buffer toggle that switches the flowing solution over the sensor surface.
The evanescent field that surface plasmons generate penetrates only a few hundred nanometers into solution, roughly a thousand times thinner than a human hair, yet within that razor-thin zone SPR instruments can detect the binding of biomolecular layers only a few angstroms thick, sensitive enough to resolve single-digit-percent changes in surface coverage.
This simulator demonstrates how surface plasmon resonance responds in real time to molecular binding events at a gold sensor surface within the Kretschmann prism-coupling configuration, showing how the reflectance dip angle shifts as analyte molecules associate with and dissociate from immobilized receptors, and how these shifts trace out a realistic sensorgram curve.
This simulator demonstrates how surface plasmon resonance responds in real time to molecular binding events at a gold sensor surface within the Kretschmann prism-coupling configuration, showing how the reflectance dip angle shifts as analyte molecules associate with and dissociate from immobilized receptors, and how these shifts trace out a realistic sensorgram curve.
Adjust the angle of incident light to locate the resonance dip in the reflectance curve, then start an analyte injection to watch the resonance angle shift as binding accumulates on the gold surface. Switch back to buffer flow to observe dissociation, and vary analyte concentration or the association and dissociation rate constants to see how they reshape the resulting sensorgram trace.
The evanescent field that surface plasmons generate penetrates only a few hundred nanometers into solution, roughly a thousand times thinner than a human hair, yet within that razor-thin zone SPR instruments can detect the binding of biomolecular layers only a few angstroms thick, sensitive enough to resolve single-digit-percent changes in surface coverage.