Metagenomics Pipeline
Results
Reads classified
0
Taxa detected
0 / 0
Community accuracy (1 − Bray–Curtis)
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Sample composition
How this pipeline works
  • Sample: a mixed pool of DNA fragments from every organism present in the environment, in proportion to how abundant that organism actually is.
  • Shotgun sequencing: the pool is cut into short reads; each read is drawn at random, so rare organisms contribute few reads and common ones contribute many.
  • Classification / binning: each read is assigned to a taxon (OTU) by sequence similarity. Similarity scoring is imperfect, so a fraction of reads land in a neighboring bin.
  • Abundance estimate: the size of each bin, divided by total reads, estimates that taxon's relative abundance. Low depth under-samples rare taxa (some may get zero reads and go undetected); more reads converge the estimate toward the true composition.